Identification of Canine Visceral Leishmaniasis in a Previously Unaffected Area by Conventional Diagnostic Techniques and Cell-block Fixation
نویسندگان
چکیده
After the report of a second case of canine visceral leishmaniasis (CVL) in São Bento da Lagoa, Itaipuaçu, in the municipality of Maricá, Rio de Janeiro State, an epidemiological survey was carried out, through active search, totaling 145 dogs. Indirect immunofluorescence assay (IFA), enzyme-linked immunosorbent assay (ELISA), and rapid chromatographic immunoassay based on dual-path platform (DPP) were used to perform the serological examinations. The parasitological diagnosis of cutaneous fragments was performed by parasitological culture, histopathology, and immunohistochemistry. In the serological assessment, 21 dogs were seropositive by IFA, 17 by ELISA, and 11 by DPP, with sensitivity of 66.7%, 66.7% and 50%, and specificity of 87.2%, 90.2% and 94%, respectively for each technique. The immunohistochemistry of bone marrow using the cell-block technique presented the best results, with six positive dogs found, three of which tested negative by the other parasitological techniques. Leishmania sp. was isolated by parasitological culture in three dogs. The detection of autochthonous Leishmania infantum in Itaipuaçu, and the high prevalence of seropositive dogs confirm the circulation of this parasite in the study area and alert for the risk of expansion in the State of Rio de Janeiro.
منابع مشابه
A Leishmania infantum FML-ELISA for the Detection of Symptomatic and Asymptomatic Canine Visceral Leishmaniasis in an Endemic Area of Iran
Background: Visceral leishmaniasis (VL) is caused by Leishmania infantum in Mediterranean basin and is an endemic disease in some parts of Iran. Canines are the main reservoirs of VL in most of the endemic areas. Different serological methods have been introduced for diagnosis of canine visceral leishmaniasis (CVL). Objective: In this survey a Fucose-Mannose Ligand (FML) ELISA, using native L. ...
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Seroprevalence of canine visceral leishmaniasis in asymptomatic dogs in Iran
Clinically infected dogs have been identified as the main reservoir hosts of visceral leishmaniasis (VL)caused by Leishmania infantum. Recently asymptomatic infected dogs were regarded to be as important asclinically ill dogs. This study was aimed to determine the seroprevalence of L. infantum infection amongasymptomatic dogs in the southwest and central parts of Iran and to investigate possibl...
متن کاملField Trial of Autoclaved Leishmania Vaccines for Control of Canine Visceral Leishmaniasis in Meshkin-Shahr, Iran
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[Canine Visceral Leishmaniasis: diagnostic tests do not detect real state of the infection].
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عنوان ژورنال:
دوره 58 شماره
صفحات -
تاریخ انتشار 2016